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Thread: TIRF Angle in CZI

  1. #1
    Junior Member
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    TIRF Angle in CZI

    I am having a problem reading the TIRF angle parameter from the CZI file.
    The image was aquired with a TIRF angle of about 77 but if I read the XML part from the CZI-file (for example with fiji) it shows:

    <TirfAngle>1.54</TirfAngle>

    What does this parameter mean and how can I convert it in degrees?

    Best regards

  2. #2
    CZ Administrators Carl Zeiss Microscopy 3's Avatar
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    Jan 2013
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    Hello JochenSeebach,

    we checked your case but cannot reproduce it.

    Please have a look at the slider

    Slider.jpg

    and the metadata

    Metadata.jpg

    of 'our' TIRF Image.

    I hope this helps to find the correct TirfAngle in the metadata.
    Last edited by Carl Zeiss Microscopy 3; 02-15-2017 at 10:09 AM.

  3. #3
    Junior Member
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    Dec 2016
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    Thanks for the response, but I still cant find the correct angle.
    If I use ZEN black to open the image, it shows indeed the correct angle of 77 (see attached screenshot), together with the correct parameters for the collimator, Laser power etc..
    However, if read the meta data for example with Fiji/ImageJ or the XML-part of the CZI-File (see text files), i can find all the other parameters, but the TIRF angle reads 1.54 instead of 77.

    So I think that the ZEN software does not not show directly the value that is stored in the Metadata (1.54), but somehow converts it, as it "converts" for examle the value the laser power in the metadata (0.6) to 60%.

    I hope this makes the problem a little bit clearer..
    Attached Images Attached Images
    Attached Files Attached Files

  4. #4
    CZ Administrators Carl Zeiss Microscopy 3's Avatar
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    Jan 2013
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    Hello JochenSeebach,

    I am very sorry but this is a forum for OAD on basis of ZEN blue only.
    Please contact your local Zeiss representative for further details.

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