The use of 3D cell culture models has emerged as a fundamental tool throughout various life science disciplines including tumor and translational biology, developmental and basic cell biology.
Elucidation of cell composition in 3D However, use of microscopy with 3D cell cultures bring new experimental challenges, including:
- Previously common analytics become more complicated when adding a third dimension. Some examples are: tracing of individual events, marker-based signal quantification, large throughputs in time-series or end-point measurements.
- Three-dimensional cell cultures are more fragile, making identification and quantification of cellular and compartmental events difficult.
- Special supports with thick well bottoms, non-planar u-bottom shapes or very high skirt heights are often necessary, which are challenging for most optical devices as well as automation routines and analysis algorithms.
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This application note presents three experimental workflows based on the automated live cell imaging microscope ZEISS Celldiscoverer 7, which overcome these challenges working with three-dimensional cell cultures. Specifically:
- Analysis of cellular responsiveness, signal transduction mechanisms, sensitivity and/or specificity towards compounds
- Monitoring of the self-organization of embryonic stem cell aggregates
- Unraveling the composition of three-dimensional cell aggregates
Quantitative Analysis of 3D Cell Cultures appeared first on Microscopy.