Hello. I am trying to figure out whether there is a way to navigate from a 20x or a 40x image to a big tiled image. I am imaging the central amygdala at 20x and I need to be able to verify that I am in the correct region. I know I can use tiles to take a whole brain image and then use more tiles to zoom in to the correct region but I am not able to save the ROI lines on the newly acquired image for some reason. I would like to do 2 thing: save the ROI that I mark on the tiled preview image on the acquired ROI image, and be able to show where the zoomed in ROI image is on the big tiled image postimaging.
I have used a camera based Keyence microscope before and on that microscope if you take a 4x image of the whole brain slice and a 40x image of the desired location, you can then go back and tell the program to mark on the 4x image where the 40x image was taken from. I would love to be able to do that on Zen.
Navigation question
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Re: Navigation question
Hello Mariam Melkumyan,
this is a forum primarily for Open Application Development (OAD)!
You can now draw the ROI in your (tiled) image. Make a right mouse click on the ROI and select 'Create Subset Images from ROI'.
Now a 'new image' is generated that corresponds to the ROI and you just have to save it.
I hope this helps.
this is a forum primarily for Open Application Development (OAD)!
In the Menu choose 'Graphics' and then 'Draw Region of Interest'.save the ROI that I mark
You can now draw the ROI in your (tiled) image. Make a right mouse click on the ROI and select 'Create Subset Images from ROI'.
Now a 'new image' is generated that corresponds to the ROI and you just have to save it.
Via OAD it is possible to convert the ROI into a Rectagle so that the Rectangle is saved with the overview image.show where the zoomed in ROI image is on the big tiled image
I hope this helps.